Journal: Bioactive Materials

Article Title: Highly photostable nanogels for fluorescence-based theranostics

doi: 10.1016/j.bioactmat.2017.03.001

Figure Lengend Snippet: Monomers used in the synthesis of PBPLP polymer.

Article Snippet: A) Excitation and emission spectra of PBPLP polymer in water, B) Emission spectra of PBPLP polymer and nanogels in solution, C) Intensity-absorbance curve of PBPLP nanogels for quantum yield measurement, and D) Fluorescent micrograph of PBPLP nanogels obtained by cytoviva (scale bar = 500 nm).

Techniques: Polymer

Journal: Bioactive Materials

Article Title: Highly photostable nanogels for fluorescence-based theranostics

doi: 10.1016/j.bioactmat.2017.03.001

Figure Lengend Snippet: Schematics of RGD functionalized, DOX loaded, fluorescence emitting crosslinked PBPLP nanogels. (Step 1) Photocrosslinkable fluorescent polymer (PBPLP) crosslinked into fluorescent nanogels in the presence of a photo-initiator, acrylic acid, and UV light; (Step 2) PBPLP nanogels can be surface functionalized with biologically active molecules (herein, RGD peptide) using EDC/sulfo-NHS chemistry; (Step 3) Doxorubicin (anti-cancer drug) can be encapsulated within these soft particles to create surface functionalized, fluorescence emitting drug delivery vehicles.

Article Snippet: A) Excitation and emission spectra of PBPLP polymer in water, B) Emission spectra of PBPLP polymer and nanogels in solution, C) Intensity-absorbance curve of PBPLP nanogels for quantum yield measurement, and D) Fluorescent micrograph of PBPLP nanogels obtained by cytoviva (scale bar = 500 nm).

Techniques: Fluorescence, Polymer

Journal: Bioactive Materials

Article Title: Highly photostable nanogels for fluorescence-based theranostics

doi: 10.1016/j.bioactmat.2017.03.001

Figure Lengend Snippet: A) Particle size distribution of PBPLP nanogels recorded by Nanosight LM20, spectrum in the inset represents the 3D plot of particle population resolved in terms of relative intensity and particle concentration. B) micrograph of nanogel morphology observed under TEM (scale bar = 200 nm), C) Nanogel size stability evaluation in PBS (pH 7.4) for a two week time period, and D) Zeta potential evaluated by a zeta potential analyzer.

Article Snippet: A) Excitation and emission spectra of PBPLP polymer in water, B) Emission spectra of PBPLP polymer and nanogels in solution, C) Intensity-absorbance curve of PBPLP nanogels for quantum yield measurement, and D) Fluorescent micrograph of PBPLP nanogels obtained by cytoviva (scale bar = 500 nm).

Techniques: Concentration Assay, Zeta Potential Analyzer

Journal: Bioactive Materials

Article Title: Highly photostable nanogels for fluorescence-based theranostics

doi: 10.1016/j.bioactmat.2017.03.001

Figure Lengend Snippet: A) Excitation and emission spectra of PBPLP polymer in water, B) Emission spectra of PBPLP polymer and nanogels in solution, C) Intensity-absorbance curve of PBPLP nanogels for quantum yield measurement, and D) Fluorescent micrograph of PBPLP nanogels obtained by cytoviva (scale bar = 500 nm).

Article Snippet: A) Excitation and emission spectra of PBPLP polymer in water, B) Emission spectra of PBPLP polymer and nanogels in solution, C) Intensity-absorbance curve of PBPLP nanogels for quantum yield measurement, and D) Fluorescent micrograph of PBPLP nanogels obtained by cytoviva (scale bar = 500 nm).

Techniques: Polymer

Journal: Bioactive Materials

Article Title: Highly photostable nanogels for fluorescence-based theranostics

doi: 10.1016/j.bioactmat.2017.03.001

Figure Lengend Snippet: A) Cytocompatibility of PBPLP nanogels against PC3 cells, B-C) Fluorescent micrograph of PC3 cells labeled with PBPLP nanogel after 8 h of incubation at 1000 μg/ml at 5× (B) and 20× magnification (C).

Article Snippet: A) Excitation and emission spectra of PBPLP polymer in water, B) Emission spectra of PBPLP polymer and nanogels in solution, C) Intensity-absorbance curve of PBPLP nanogels for quantum yield measurement, and D) Fluorescent micrograph of PBPLP nanogels obtained by cytoviva (scale bar = 500 nm).

Techniques: Labeling, Incubation

Journal: Bioactive Materials

Article Title: Highly photostable nanogels for fluorescence-based theranostics

doi: 10.1016/j.bioactmat.2017.03.001

Figure Lengend Snippet: A) Particle size distribution of PBPLP nanogels. B) Drug release profiles of PBPLP/DOX nanogels in 0.1 M acetic acid (pH 5.2) and 0.1 M phosphate buffer (pH 7.4). C) Pharmacological activity of PBPLP/DOX against prostate cancer cell lines (PC3). Figure legend: Media = pure media (negative control); PBPLP = 50 μg/ml nanogel (DOX-free control); PBPLP(DOX1, 2, or 3) = 5, 25, or 50 μg/ml respectively of nanogel loaded with 20 wt% of DOX; DOX = 10 μg/ml DOX (positive control). D) Light microscopy of PC3 cells incubated with PBPLP/DOX3 at 12 h (top left), 24 h (right), and 48 h (bottom left).

Article Snippet: A) Excitation and emission spectra of PBPLP polymer in water, B) Emission spectra of PBPLP polymer and nanogels in solution, C) Intensity-absorbance curve of PBPLP nanogels for quantum yield measurement, and D) Fluorescent micrograph of PBPLP nanogels obtained by cytoviva (scale bar = 500 nm).

Techniques: Activity Assay, Negative Control, Control, Positive Control, Light Microscopy, Incubation

Journal: Bioactive Materials

Article Title: Highly photostable nanogels for fluorescence-based theranostics

doi: 10.1016/j.bioactmat.2017.03.001

Figure Lengend Snippet: Cellular internalization of PBPLP/DOX and delivery of DOX to the nucleus. Bright field and fluorescent micrograph of cellular uptake for (A) PBPLP nanogels, (B) PBPLP/DOX (50 μg/ml), and (C) free DOX (1 μg/ml) at 24 h. PBPLP nanogels stain the cytoplasm and free or released DOX stains cell nuclei. Scale bar 50 μm.

Article Snippet: A) Excitation and emission spectra of PBPLP polymer in water, B) Emission spectra of PBPLP polymer and nanogels in solution, C) Intensity-absorbance curve of PBPLP nanogels for quantum yield measurement, and D) Fluorescent micrograph of PBPLP nanogels obtained by cytoviva (scale bar = 500 nm).

Techniques: Staining

Journal: Human molecular genetics

Article Title: The fragile X mental retardation protein is a ribonucleoprotein containing both nuclear localization and nuclear export signals.

doi: 10.1093/hmg/5.8.1083

Figure Lengend Snippet: Figure 1. Subcellular localization of FMRP constructs transiently transfected into COS-7 cells. (A) Full-length FMRP, Iso4, and carboxyl-terminal deletion constructs with amino acid numbers labeled according to Ashley et al. (13). The percentage of cells that displayed nuclear staining is indicated with n = the number of cells exhibiting fluorescent staining (e.g. 99% of the cells transfected with Iso4 showed predominant nuclear staining while the remainder of the cells showed no detectable staining in the nucleus). (B) Fluorescent micrographs showing the subcellular localization of FMRP constructs in transiently transfected COS-7 cells via indirect immunofluorescent staining with the anti-FLAG M2 antibody. The transfected FMRP constructs are identified in each panel.

Article Snippet: Fluorescent micrographs were taken with Kodak EL 400 film using identical exposure times.

Techniques: Construct, Transfection, Labeling, Staining

Journal: Human molecular genetics

Article Title: The fragile X mental retardation protein is a ribonucleoprotein containing both nuclear localization and nuclear export signals.

doi: 10.1093/hmg/5.8.1083

Figure Lengend Snippet: Figure 2. Subcellular localization of CMPK-FMRP amino terminus fusion proteins. (A) FMRP amino terminus fusion constructs with chicken muscle pyruvate kinase (CMPK, amino acids 17-476). The percentage of cells that displayed nuclear staining is indicated with n = the number of cells displaying fluorescent staining. (B) Micrographs showing subcellular localization of FMRP-CMPK fusion constructs in COS-7 cells with the transfected constructs identified in each panel. (C) FMRP residues 1–184 with basic residues in black boxes. Possible NLS containing region is underlined.

Article Snippet: Fluorescent micrographs were taken with Kodak EL 400 film using identical exposure times.

Techniques: Construct, Staining, Transfection

Journal: Human molecular genetics

Article Title: The fragile X mental retardation protein is a ribonucleoprotein containing both nuclear localization and nuclear export signals.

doi: 10.1093/hmg/5.8.1083

Figure Lengend Snippet: Figure 3. Subcellular localization of FMRP constructs with exon 14 deletions. (A) FMRP constructs with carboxyl terminal truncations of exon 14-encoded residues. The remaining residues encoded by exon 14 are indicated in ∆441 and ∆432. ∆17 corresponds to full-length FMRP with residues 425–441 removed. The percentage of cells that displayed nuclear staining is indicated with n = the number of cells exhibiting fluorescent staining. (B) Micrographs showing subcellular localization of exon 14 deletion constructs in COS-7 cells with the transfected FMRP constructs identified in each panel.

Article Snippet: Fluorescent micrographs were taken with Kodak EL 400 film using identical exposure times.

Techniques: Construct, Staining, Transfection

Journal: Human molecular genetics

Article Title: The fragile X mental retardation protein is a ribonucleoprotein containing both nuclear localization and nuclear export signals.

doi: 10.1093/hmg/5.8.1083

Figure Lengend Snippet: Figure 4. Delineation of the FMRP nuclear export signal. (A) FMRP fusion constructs with CMPK. The percentage of cells that displayed nuclear staining is indicated with n = the number of cells exhibiting fluorescent staining. (B) Micrographs showing subcellular localization of FMRP-CMPK fusion constructs in COS-7 cells with the transfected constructs identified in each panel. (C) Amino acid alignment of NES sequences of Rev, PKI, and FMRP with identical residues in dark blue and similar residues in light blue. Sequences aligned with the Clustal W program using the blosum weight matrix. (D) NES and M1 peptides conjugated to BSA. The putative NES sequence is underlined. In M1, black boxes indicate residues that were mutated from leucine to alanine. (E) Micrographs of COS-7 cells 1 h after nuclear injection of BSA-peptide conjugates. FITC-labeled BSA-NES (green) and LRSC-labeled IgG (red) coinjected and incubated at 37C. BSA-NES injected and incubated at 4C. BSA-M1 injected and incubated at 37C.

Article Snippet: Fluorescent micrographs were taken with Kodak EL 400 film using identical exposure times.

Techniques: Construct, Staining, Transfection, Sequencing, Injection, Labeling, Incubation